Blog

Body Fluids Analysis with Linearity Down to Zero

Posted on by admin
Body Fluids Analysis with Linearity Down to Zero
30
Jun

Why Test Body Fluids?

Body fluid analysis is a challenging task for most labs because the samples—such as cerebrospinal, synovial, pleural fluid, pericardial, peritoneal and general serous fluid are difficult to draw– not to mention a discomforting experience for the patient.

However, it’s an imperative part of diagnosis, as fluid analysis assists in the identification of certain disease states including meningitis, hemorrhage, malignancy, inflammation, viral, bacterial/parasitic infections and more. Because urgent diagnostic data is often sought after from even a very limited sample for the diagnosis of these disease states, accuracy and speed of results take on even greater importance.

For this reason, laboratories have traditionally resorted to manual body fluid cell counting to ensure the highest quality of insights. However, lately there’s been a shift to an automated approach, including analyzer-based body fluid analysis.

How Body Fluids are Analyzed

Body fluid analysis is performed in one of two overall ways: manual cell count using a hemocytometer and an automated approach using an automated analyzer.

While manual counting is the gold standard, there are numerous downsides to the process, as it is time consuming, taking at least 30 minutes to an hour to perform a total RBC and WBC count. Manual review requires a well-trained, licensed personnel due to the testing complexity.

It’s also a subjective approach where competency and technique of testing personnel are not always consistent. Lastly, it’s a labor-intensive process because it requires the staff’s full attention and they are unable to perform any other tasks.

After all this manual work, results must be calculated by hand and this can potentially cause clerical and mathematical errors– even with the use of a calculator.

How Automated Body Fluid Testing is Different

Automated counting removes many of the variables involved with manual review. Foremost, it’s time saving because it takes about five minutes to perform the total RBC and WBC count. It’s moderately complex to perform and can be done by any licensed personnel.

It also reduces the potential for user subjectivity and requires minimal labor. Unlike manual counting, it increases productivity of testing personnel and frees up staff so that they can perform other laboratory tasks.

There are three methods to automated cell counting:

  • Method 1: Enhanced Impedance counting is available on some hematology platforms and relies on the displacement of electrical field to provide information on particle size and volume. This aids in classifying particles.
  • Method 2: Simple Flow Cytometry measures cell granularity, size and light scatter. This can be performed on some hematology platforms. The laser detects size, volume and shape that aides in classifying particles.
  • Method 3: Particle and Image Recognition used by instruments like the iQ200 automated urine microscopy analyzer. The cells are counted by digital flow morphology, allowing the system to capture actual images of particles and classifies by size, shape, contrast and texture. A visual inspection by a technician can be done after analysis is complete, if they need to identify real cells from artifacts.

Do Different Automated Methods Have the Same Functional Sensitivity?

The functional sensitivity for the above-mentioned methods is not the same. For example, in Method #3, the particle and image recognition method offer low count sensitivity and linearity. It can even go down to zero for RBC and WBC.

Also, limitations at the zero of low count range narrows the application of automation for all types of body fluids, particularly clear/colorless CSF. Normally, counting this kind of fluid can only be done by the labor-intensive manual method or by using the iQ200 automated analyzer (Method #3).

However, all the listed automated methods are far less subjective and produce faster results than the gold standard manual method by removing the need for manual counting.

Additionally, when using an automated approach, body fluids with very large cells or cells clumped together like tumor cells cannot be accurately counted by the first two methods. They can be identified by the iQ200 automated analyzer (Method #3) due to the instrument’s ability to identify and capture images of particle during the analysis. iQ200 technicians visually inspect particle images, and when they see very large or clumped tumor cells on the iQ200, the technician is alerted to check test results in hematology for these cells when performing WBC differential counts.

Body fluids analysis module

 

Below is a summary of the benefits of an automated approach when it comes to testing body fluids versus a manual approach:

Manual

  • Most body fluid specimens do not need to be diluted prior to plating on the hemacytometer
  • Dilutions are estimated based on appearance and are “hit or miss” at being correct the first time
  • Time-consuming and labor-intensive to count cells manually
  • Resulting count is completely subjective-even in the most disciplined labs
  • Requires extensive expertise which may vary from tech-to-tech
  • Lab regulations require counting cells twice per sample
  • Requires complete attention of the technologist

Automated (benefits)

  • Counting is streamlined
  • Dilutions are standardized according to specimen color and clarity (no hit or miss)
  • Provides exact directions for performing dilutions
  • Resulting count is less subjective leading to less variation tech to tech
  • Sample processing is easier than manual methods
  • Requires expertise, but much less extensive
  • Cells are only counted once
  • Techs are free to run other instruments while the body fluid is processed

Source: Body Fluids Analysis with Linearity Down to Zero Webinar, 2017.

Better Body Fluids Analysis with Automated Urine Microscopy Analyzer

For laboratories looking to reduce manual work associated with manual review, there is a better solution. The iQ200 series automated urine microscopy analyzer can provide digital images and shorten turn-around-time with standardized results.

With the optional Body Fluid Module, laboratories can be empowered with a complete body fluids menu and on-screen particle isolation. Some of the benefits of the Body Fluids Module with use of synovial fluids are listed in the table below:

Benefits

 Body Fluids Module with Synovial Fluids Other Methods

Performance

 Digital images; standardization; broad reportable range to zero Manual: prone to technical error; subjective
Automated: less broad reportable range

Productivity

 Faster TAT; can run urine and body fluid samples together without
segregation; minimal volume		 Manual: labor-intensive
Other automated system: specimens must be segregated

Value

 Reduces the need for “send outs”; reduces analysis time;
walkaway convenience allows techs to perform other duties		 Manual: not walkway
Other automated systems: not walkaway

Source: Body Fluids Analysis with Linearity Down to Zero Webinar, 2017.

According to Pat Guinane, MT(ASCP), core laboratory supervisor at Arnot Ogden Medical Center in Elmira, New York, there are a host of benefits to digital flow morphology for body fluids as opposed to using a hemacytometer. The most notable being improved day-to-day workflow.

“Digital flow morphology for body fluids analysis is a win-win situation,” Pat said, “before the iQ200, we performed most body fluids on our hematology analyzer, except synovial fluids – which were manual counts due to the viscosity of the specimen. Many times, they were below linearity of the hematology analyzer so we would end up doing manual counts.” Pat pointed out that body fluids often arrive at inconvenient times. “For example, during shift change, as well as the off-shifts,” she noted. “The iQ200 has streamlined our workflow immensely during the off-shifts.”

In practice, Pat has also achieved standardization of body fluid counting and resulting by using the iQ while minimizing tech-to-tech variation. “It’s what the iRICELL does, it’s almost the definition of the Iris,” she noted.

The iQ200 has also greatly improved turn-around-time for body fluids results in this laboratory, according to Laura Wintjen, CLS (ASCP), core laboratory lead technologist at Arnot Ogden. “This has freed techs up to do other tasks within the lab,” she added.

Overall, using the body fluids module improved employee satisfaction by reducing time spent at a microscope. “Everyone would agree that the Iris has really been a game changer,” Pat said.

While Argot Ogden’s lab has experienced stable volume since automating, it’s affiliated with Lake Erie College of Osteopathic Medicine and sometimes ordering habits of physicians are not consistent. Therefore, it does make it easier to keep up with varying numbers of orders, especially when it comes to body fluids.

Physicians are also happy with getting a low and accurate count on their body fluids and if there is question regarding any data, medical technologist can easily pull them up and review the images. “This is also beneficial regarding competencies,” Laura said. “If the image is reviewed due to a doctor’s request and it’s correct, we can use that for a competency of the tech.”

Lastly, the benefit of images on the iQ200 is especially helpful. “Images are always the same, you don’t have to worry about lighting and the diaphragm.” Laura explained. “There are no problems with refraction due to improper illumination or plane of focus.”

Images also help with streamlining training and competencies with techs. “We don’t have to wait for certain crystals or other infrequent sediment to show up in order to get techs proficient in the identification,” Laura said. “We do a monthly check of body fluids on the instrument to look at any abnormal cells and randomly select staff to review for identification of competencies.”

Images are also useful in comparing discrepancies when a physician may call and question results. The images can be pulled up and verified or amended as well as follow-up with techs.

All in all, there are numerous benefits to automating body fluid analysis. It is one sure way to simplify a challenging task that is an essential part of diagnosing very serious medical conditions. Since urgent diagnostic data is often sought after from even a very limited sample for the diagnosis of these disease states, accuracy and speed of results take on even greater importance. Learn more about streamlining the process in your lab with our automated body fluids solution and move your body fluid microscopy forward.

admin

93 thoughts on “Body Fluids Analysis with Linearity Down to Zero”

  1. Code of your destiny says:

    I’m really inspired together with your writing skills and also with the layout to your blog. Is that this a paid topic or did you modify it yourself? Either way stay up the nice quality writing, it’s uncommon to look a nice weblog like this one today!

  28. Pingback: buy generic zoloft

  29. Pingback: cipro for uti

  30. Pingback: uti antibiotics online

  33. Pingback: fluconazole over the counter canada

  35. Pingback: diflucan over the counter canada

  40. Pingback: vidalista black review

  41. Pingback: cenforce 25 vs viagra

  43. Pingback: loperamide doses

  44. Pingback: cialis dosage options

  49. Pingback: finasteride enlarged prostate

  51. Pingback: tirzepatide vs semaglutide cost reddit

  53. Pingback: semaglutid tablett reseptfritt

  54. Pingback: wegovy semaglutida é ozempic

  55. Pingback: semaglutid tablett viktnedgång

  56. Pingback: rogaine minoxidil beard

  57. Pingback: doxycycline tablet 100mg for dogs

  59. Pingback: flagyl for bv dose

  62. Pingback: minoxidil reviews female

  63. Pingback: lowest dose of bupropion sr

  67. shkola onlain_buOi says:

    Честно говоря, долго выбирал, куда поступать, но после советов хороших знакомых наткнулся на один действительно толковый вариант. Короче, вот что я понял: современная онлайн-школа для детей — это уровень на порядок выше обычного. Там и преподаватели живые и вовлеченные, и дети занимаются с реальным интересом.

    В общем, кому реально нужно нормальное обучение в теме образовательные онлайн школы — убедитесь во всём сами, вот здесь все расписано в деталях: online school online school.

    Думаю, это как раз то, что сейчас нужно многим родителям. Потому что стандартный дистант бывает дико скучным для ребенка, а тут организована именно живое регулярное общение с кураторами. Пригодится точно, потом еще спасибо скажете.

  68. vivod iz zapoya v stacionare_pyka says:

    Всем доброго времени суток. Дело деликатное, но решил черкануть пару строк, так как в сети сейчас полно сомнительных клиник. Когда нужен проверенный и опытный врач для капельницы, лучше сразу обращаться к сертифицированным медикам.

    Знакомые вызывали бригаду в похожей ситуации в итоге вся ценная информация была собрана по крупицам. Если вам актуально или ситуация экстренная, вся информация есть здесь: выведение из запоя в стационаре выведение из запоя в стационаре.

    На этом ресурсе действительно дана полная информация, и помощь окажут полностью конфиденциально. Надеюсь, эта рекомендация и обращайтесь к настоящим профессионалам. Всем душевного спокойствия!

  69. melbet_ylPt says:

    Давно искал инфу и наконец-то нашел нормальный разбор темы. Всё расписано до мелочей, даже новичок поймет что к чему. Многие на форумах спорят, а ответ лежал на поверхности. Вот melbet melbet — переходите, там вся суть. Там внутри и примеры, и пошаговые инструкции, короче полный фарш.

  70. syvenirnaya prodykciya s logotipom_yzpi says:

    Срочно нужен совет кто уже заказывал партию к выставке. Планируем раздачу для партнёров на новый год. Везде говорят про индивидуальный подход, но реально толковое изготовление корпоративных сувениров с печатью по вменяемой цене. корпоративные подарки клиентам с логотипом https://suvenirnaya-produkcziya-s-logotipom-9.ru Интересует именно изготовление под ключ — от кружек до брендированных блокнотов. Нам нужно от 500 штук, можно меньше. Заранее спасибо, кто откликнется.

  71. 1xbet giris_oyEi says:

    Şu bahis işlerine merak salalı çok oldu. Herkes bir şey diyor ama kimse net konuşmuyor. Adımları doğru sırayla uyguladıktan sonra bağlantı hatasız açıldı. En sonunda güvenilir bir kaynak buldum ve size de aktarayım dedim: 1xbet spor bahislerinin adresi 1xbet spor bahislerinin adresi. Ne diyeyim yani anlatayım mı — bu işin ehli belli başlı yani.

    çekimler konusunda da sıkıntı yok yani rahat olun. Kendi adıma konuşuyorum size açık açık — pişman olacağınızı sanmıyorum hiç deneyin derim. Hayırlı olsun herkese diliyorum…

  73. 1xbet giris_oqkn says:

    Daha önce hiç böyle bir site görmemiştim. Açıkçası önyargılıydım biraz. Sonra biraz araştırayım dedim.

    Spor bahislerinde iddialı olanlar buraya. Detaylı incelemeleri tamamlayıp adımları takip ettikten sonra her şey netleşti. Giriş adresi işte karşınızda: 1xbet güncel 1xbet güncel. Kısacası durum ortada — 1xbet spor bahislerinin adresi burada.

    Hem hızlı hem güvenilir. Kendi adıma konuşuyorum — başka bir yere ihtiyacınız kalmaz. Şimdiden iyi eğlenceler…

  74. Narkolog na dom_woOi says:

    Вот реально ситуация — человек в ступоре , а везти в клинику просто нереально . Я сам через это прошел недавно совсем. Сидишь, не знаешь за что хвататься . Начинаешь обзванивать знакомых , а вокруг сплошной развод . Пока кто-то не подсказал один нормальный проверенный вариант. Требуется срочная помощь — а везти самому просто нереально, то выход один . Я про наркологическую помощь на дому . У нас в Самаре, к слову , хватает левых контор без лицензии. Вся проверенная информация вот тут : нарколог на дом анонимно нарколог на дом анонимно Откровенно говоря, после того как прочитал , понял, как правильно действовать. И про снятие запоя на дому, и про последующее кодирование. Плюс анонимность — это важно . Советую не откладывать.

  75. Narkolog na dom_ajOi says:

    Вот реально ситуация — родственник в тяжелом запое , а тащить в больницу страшно . Моя семья такое пережила пару лет назад . Руки опускаются, время тикает. Лезешь в интернет, а вокруг одни обещания . Пока случайно не нашел один нормальный проверенный вариант. Если нужна немедленная консультация — а ехать куда-то нет физической возможности , то нужно вызывать врача на дом. Речь конкретно про наркологическую помощь на дому . У нас в Самаре, к слову , тоже полно левых контор без лицензии. Нормальные контакты, кто реально приезжает ниже по ссылке: нарколог домой нарколог домой Честно скажу , после того как вник в детали, понял, как правильно действовать. И про снятие запоя на дому, и про последующее кодирование. Плюс анонимность — это важно . Советую не тянуть .

  76. tkan dlya mebeli_kspt says:

    Если честно, сам перерыл кучу форумов в поисках нормальной обивки. Оказалось, что выбрать подходящий вариант реальная проблема. Короче, смотрите, вот здесь реально толково расписано про плотность, ворс и износостойкость для диванов и кресел, а главное — показаны варианты, которые не выцветают. Вся полезная информация доступна здесь: ткань для диванов https://tkan-dlya-mebeli-2.ru Дальше сами гляньте примеры в интерьере. Да, и не берите первое, что попалось — я уже поплатился кошельком, когда брал ткань для мебели на распродаже. Эта тема реально вывозит по соотношению цена-качество. Кстати: ткань для обивки мебели купить лучше уже с нормальной пропиткой от грязи. Да и трётся такое полотно гораздо меньше. Здесь реально дельные советы.

  77. binance says:

    I don’t think the title of your article matches the content lol. Just kidding, mainly because I had some doubts after reading the article.

  78. luxury car rental miami_wlPn says:

    I’ve got the scars to prove it, the rental landscape down here is crazy. Then you show up at the local lot to pick up the car. Different car waiting — scratches everywhere, smells like an ashtray, and that “amazing price”? Doesn’t include the mandatory $400 cleaning fee or the $30 per day toll pass you can’t waive. Eight years in South Florida and these clowns still almost get me. When you need a proper and reliable premium ride to cruise around, run far from the airport counters. Miami without decent wheels is basically a hostage situation, whether you are doing South of Fifth brunch, Design District shopping, or a spontaneous Keys trip.

    I’ve run through maybe 45 rental companies across Dade, Broward, and Monroe, until I finally found one outfit that doesn’t play stupid games. If you are looking for the only honest source for premium wheels across South Florida, check the current details here: rent a sedan car rent a sedan car. Yeah, parking in South Beach will cost you a nice bottle of wine — but that’s the Miami tax. Just drive safe out there and absolutely skip that “windshield protection” upsell — pure profit for them, zero value for you. hope this helps some of you save a few bucks.

  79. luxury car rental miami_tqPn says:

    Alright, real talk about the Miami rental game — it’s a straight-up jungle out here. Then you show up at the local lot to pick up the car. Different car waiting — scratches everywhere, smells like an ashtray, and that “amazing price”? Doesn’t include the mandatory $400 cleaning fee or the $30 per day toll pass you can’t waive. Eight years in South Florida and these clowns still almost get me. When you need a proper and reliable premium ride to cruise around, do some real digging first and read actual customer reviews. Miami without decent wheels is basically a hostage situation, especially since the AC must be arctic cold and unlimited miles non-negotiable.

    Most of these local agencies are just shiny websites hiding the same beat-up fleet with fake reviews, but I eventually found a service where what you book is exactly what shows up, no surprises, no fine print nightmares. If you are looking for the only honest source for premium wheels across South Florida, check the current details here: miami exotic car rental miami miami exotic car rental miami. Yeah, parking in South Beach will cost you a nice bottle of wine — but that’s the Miami tax. Just drive safe out there and absolutely skip that “windshield protection” upsell — pure profit for them, zero value for you. hope this helps some of you save a few bucks.

  80. 1xbet indir_yfoi says:

    1xbet indir nasıl yapılır diye çok araştırdım valla. Herkes farklı bir şey tavsiye ediyordu kime güveneceğimi bilemedim. Adımları doğru sırayla uyguladıktan sonra erişim sorunsuz açıldı. En sonunda güvenilir bir kaynağa ulaştım ve size de tüm detayları aktarmak istedim, güncel bilgilere buradan bakabilirsiniz: 1xbet nouvelle version à télécharger 1xbet nouvelle version à télécharger. Yani anlatmak istediğim şu — mobil uygulaması inanılmaz hızlı ve stabil çalışıyor.

    Hiçbir sorun yaşamadım indirme işleminde. Birçok platform denedim ama en iyisi bu çıktı — başka yerde vakit kaybetmeyin yani. Umarım siz de memnun kalırsınız…

  83. luxury car rental miami_jkPn says:

    Alright, real talk about the Miami rental game — it’s a straight-up jungle out here. You find this amazing deal online: brand new Beamer, unlimited miles, price that makes you smile. Different car waiting — scratches everywhere, smells like an ashtray, and that “amazing price”? Doesn’t include the mandatory $400 cleaning fee or the $30 per day toll pass you can’t waive. Fool me eight times? That’s just another Tuesday in the 305. When you need a proper luxury car rental miami. Miami without decent wheels is basically a hostage situation. leather seats that won’t weld themselves to your thighs in July. most are shiny turds with five-star fake reviews on Google Maps. Finally found one outfit that doesn’t play stupid games. prices swing like crazy so check before the weekend rush:
    miami south beach rental cars miami south beach rental cars also bring serious shades unless you enjoy driving straight into the sun like a zombie. drive safe and absolutely skip that “windshield protection” upsell — pure profit for them, zero value for you.

  84. luxury car rental miami_xupa says:

    Alright listen up because I’m about to save you a massive headache. Miami rental game is wild — half these clowns show you a Mercedes online and hand you a busted Charger with mismatched tires. Plus the fine print says you can’t even drive to Orlando. Fool me four times? Not happening. miami luxury car rental. Miami without a decent whip is basically a punishment. leather that doesn’t glue to your legs in July heat. I’ve tested maybe 25 rental outfits across Dade and Broward. what you book is what you get, period. Here’s the only straight-up source for premium wheels in South Florida
    exotic car rental miami exotic car rental miami also bring polarized shades unless you enjoy driving blind into sunset. drive safe and maybe pass on that overpriced roadside assistance add-on.

  85. mejdynarodnie plateji_ydOi says:

    Знаете, — как найти адекватный способ перевода денег за границу онлайн. В одном блоге вычитал вот этот разбор: платежный агент за рубежом https://mezhdunarodnye-platezhi-nar.ru Главное, что нужно понять — комиссии могут сильно отличаться. Согласитесь, очередной международный перевод — это риск переплатить в два раза. Обратите внимание — до любой операции сравните условия. В противном случае легко пролететь с курсом. Резюмируя, — стоит разобраться заранее.

  87. mejdynarodnie plateji_ykpl says:

    Долго откладывал, но созрел — где брать адекватные тарифы для платежей за рубежом. Скинули ссылку в телеграме — держите, вот нормальный разбор: платежи для импортеров https://mezhdunarodnye-platezhi-tov.ru Короче, суть такая — комиссии у всех разные как с неба. Потому что любой подобный?? перевод — это риск потерять на конвертации. Вот ещё какой момент — прежде чем отправлять обязательно сравните хотя бы пару вариантов. В противном случае легко переплатить в два раза. Как итог — стоит один раз разобраться.

  88. Pingback: ketoconazole for skin infections

  89. 1xbet app_iqSt says:

    Je cherchais une application mobile pour mes paris sportifs. Je ne trouvais pas la version officielle sur le Play Store. Finalement, j’ai pris le temps d’analyser tous les détails techniques. J’ai finalement déniché la bonne source et je voulais vous partager tous les détails, vous pouvez consulter les informations à jour ici: download 1xbet apk download 1xbet apk. Bref, ce que je voulais vous dire — la dernière version est super fluide et intuitive.

    les mises à jour se font automatiquement. J’ai comparé plusieurs apps mais celle-ci est la meilleure — c’est clairement l’application la plus performante du marché. Bonne chance à tous…

  90. zakazat kyhnu_sden says:

    Народ кто в Питере живет. Объездил полгорода салонов — везде перекупы. То ручки через месяц шатаются. Короче, мужики с руками из правильного места — заказать кухню без посредников. Замер на следующий день. В общем, сохраняйте — купить кухню в спб купить кухню в спб Проверяйте по этому списку. Перешлите кому надо.

  92. kyhni SPb_knKn says:

    Питерцы отзовитесь Оббегал все салоны в городе — везде одно и то же То кромка кривая через раз Короче, единственные кто делает совестливо — заказать кухню по индивидуальным размерам Фасады на выбор из 50 цветов В общем, там цены и примеры работ — кухня глория https://kuhni-spb-ytr.ru Не ведитесь на салоны-прокладки с накруткой Перешлите другу кто тоже мучается

  93. 1xbet apk_znSt says:

    Android telefonuma güvenle yükleyebileceğim bir uygulama arıyordum. Herkes farklı bir şey tavsiye ediyordu kime inanacağımı şaşırdım. Adımları doğru sırayla uyguladıktan sonra erişim sorunsuz açıldı. En sonunda sağlam bir adrese ulaştım ve size de tüm detayları aktarmak istedim, güncel bilgilere buradan bakabilirsiniz: 1xbet yükle android 1xbet yükle android. Yani anlatmak istediğim şu — mobil versiyonu her şeyi thinkmüş resmen.

    güncellemeleri de düzenli yapılıyor. Birçok apk denedim ama en başarılısı bu çıktı — kesinlikle pişman olmazsınız deneyin derim. Umarım siz de memnun kalırsınız…

Leave a Reply

Your email address will not be published. Required fields are marked *